BlueGene Biotech Human Adenosine Triphosphate ELISA kit
E01A0038 Human Adenosine Triphosphate ELISA kit
Human Adenosine Triphosphate ELISA kit is suitable for the detection of samples from human species. Adenosine Triphosphate can also be called Adenosine-5′-Triphosphate, ATP.
Specifications of Human Adenosine Triphosphate ELISA kit
Product Information
Cat. No.
E01A0038
Product Name
Human Adenosine Triphosphate ELISA kit
Species
Human
Product Size
48 Tests / 96 Tests
Concentration
50-1000 ng/mL
Sensitivity
1.0 ng/mL
Principal
Competitive ELISA
Sample Volume
100 ul
Sample Type
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time
90 minutes
Platform
Microplate Reader
Conjugate
HRP
Detection Method
Colorimetric
Storage
2-8°C
Kit Components
MATERIALS
SPECIFICATION
QUANTITY
MICROTITER PLATE
96 wells
stripwell
ENZYME CONJUGATE
6.0 mL
1 vial
STANDARD A (0.5mL)
0 ng/mL
1 vial
STANDARD B (0.5mL)
50 ng/mL
1 vial
STANDARD C (0.5mL)
100 ng/mL
1 vial
STANDARD D (0.5mL)
250 ng/mL
1 vial
STANDARD E (0.5mL)
500 ng/mL
1 vial
STANDARD F (0.5mL)
1000 ng/mL
1 vial
SUBSTRATE A
6 mL
1 vial
SUBSTRATE B
6 mL
1 vial
STOP SOLUTION
6 mL
1 vial
WASH SOLUTION (100 x)
10 mL
1 vial
BALANCE SOLUTION
3 mL
1 vial
Principle of the Assay
ATP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ATP antibody and an ATP-HRP conjugate. The assay sample and buffer are incubated together with ATP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP concentration since ATP from samples and ATP-HRP conjugate compete for the anti-ATP antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP from the sample, fewer sites are left to bind ATP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP concentration in each sample is interpolated from this standard curve.
Quality Control on Human Adenosine Triphosphate ELISA kit
Coefficient of Variance
Intra Variation% <10%
Inter Variation% <12%
Recovery
94-106%
Linearity
Diluent Ratio
Range %
1:2
93-101
1:4
90-107
1:8
92-105
Specificity/Cross-reactivity
No significant cross-reactivity or interference between ATP and analogues was observed.
Citations of Human Adenosine Triphosphate ELISA kit
E01A0038 has been referenced in the below publications:
The research of the metabolism and in vivo functional changes of erythrocyte stored at -65℃.