BlueGene Biotech Human Adrenomedullin ELISA kit
E01A0047 Human Adrenomedullin ELISA kit
Human Adrenomedullin ELISA kit is suitable for the detection of samples from human species. Adrenomedullin can also be called AM, ADM, ADM precursor, ADML.
Specifications of Human Adrenomedullin ELISA kit
Porduct Information
Cat. No.
E01A0047
Product Name
Human Adrenomedullin ELISA kit
Species
Human
Product Size
48 Tests / 96 Tests
Concentration
100-2500pg/mL
Sensitivity
1.0 pg/mL
Principal
Competitive ELISA
Sample Volume
100 ul
Sample Type
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time
90 minutes
Platform
Microplate Reader
Conjugate
HRP
Detection Method
Colorimetric
Storage
2-8°C
Kit Components
MATERIALS
SPECIFICATION
QUANTITY
MICROTITER PLATE
96 wells
stripwell
ENZYME CONJUGATE
6.0 mL
1 vial
STANDARD A (0.5mL)
0 pg/mL
1 vial
STANDARD B (0.5mL)
100 pg/mL
1 vial
STANDARD C (0.5mL)
250 pg/mL
1 vial
STANDARD D (0.5mL)
500 pg/mL
1 vial
STANDARD E (0.5mL)
1000 pg/mL
1 vial
STANDARD F (0.5mL)
2500 pg/mL
1 vial
SUBSTRATE A
6 mL
1 vial
SUBSTRATE B
6 mL
1 vial
STOP SOLUTION
6 mL
1 vial
WASH SOLUTION (100 x)
10 mL
1 vial
BALANCE SOLUTION
3 mL
1 vial
Principle of the Assay
AM ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AM antibody and an AM-HRP conjugate. The assay sample and buffer are incubated together with AM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AM concentration since AM from samples and AM-HRP conjugate compete for the anti-AM antibody binding site. Since the number of sites is limited, as more sites are occupied by AM from the sample, fewer sites are left to bind AM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AM concentration in each sample is interpolated from this standard curve.
Quality Control on Human Adrenomedullin ELISA kit
Coefficient of Variance
Intra Variation% <10%
Inter Variation% <12%
Recovery
89-107%
Linearity
Diluent Ratio
Range %
1:2
95-102
1:4
90-108
1:8
91-112
Specificity/Cross-reactivity
No significant cross-reactivity or interference between AM and analogues was observed.
Citations of Human Adrenomedullin ELISA kit
E01A0047 has been referenced in the below publications:
Analysis of the Secretome of Apoptotic Peripheral Blood Mononuclear Cells: Impact of Released Proteins and Exosomes for Tissue Regeneration.