BlueGene Biotech Human Asymmetric Dimethylaoyoinine ELISA kit
E01A0035 Human Asymmetric Dimethylaoyoinine ELISA kit
Human Asymmetric Dimethylaoyoinine ELISA kit is suitable for the detection of samples from human species. Asymmetric Dimethylaoyoinine can also be called N-Dimethylarginine: NG, NG-Dimethylarginine dihydrochloride, Asymmetric Dimethylarginine, ADMA.
Specifications of Human Asymmetric Dimethylaoyoinine ELISA kit
Product Information
Cat. No.
E01A0035
Product Name
Human Asymmetric Dimethylaoyoinine ELISA kit
Species
Human
Product Size
48 Tests / 96 Tests
Concentration
2.5-50 μmoL/L
Sensitivity
0.1 μmoL/L
Principal
Sandwich ELISA
Sample Volume
50 ul
Sample Type
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time
90 minutes
Platform
Microplate Reader
Conjugate
HRP
Detection Method
Colorimetric
Storage
2-8°C
Kit Components
MATERIALS
SPECIFICATION
QUANTITY
MICROTITER PLATE
96 wells
stripwell
ENZYME CONJUGATE
10 mL
1 vial
STANDARD A (0.5mL)
0 μmoL/L
1 vial
STANDARD B (0.5mL)
2.5 μmoL/L
1 vial
STANDARD C (0.5mL)
5.0 μmoL/L
1 vial
STANDARD D (0.5mL)
10 μmoL/L
1 vial
STANDARD E (0.5mL)
25 μmoL/L
1 vial
STANDARD F (0.5mL)
50 μmoL/L
1 vial
SUBSTRATE A
6 mL
1 vial
SUBSTRATE B
6 mL
1 vial
STOP SOLUTION
6 mL
1 vial
WASH SOLUTION (100 x)
10 mL
1 vial
BALANCE SOLUTION
3 mL
1 vial
Principle of the Assay
ADMA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ADMA. Standards or samples are then added to the microtiter plate wells and ADMA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ADMA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ADMA are added to each well to “sandwich” the ADMA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ADMA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADMA concentration in each sample is interpolated from this standard curve.
Quality Control on Human Asymmetric Dimethylaoyoinine ELISA kit
Coefficient of Variance
Intra Variation% <10%
Inter Variation% <12%
Recovery
91-107%
Linearity
Diluent Ratio
Range %
1:2
95-102
1:4
90-107
1:8
93-109
Specificity/Cross-reactivity
No significant cross-reactivity or interference between ADMA and analogues was observed.
Citations of Human Asymmetric Dimethylaoyoinine ELISA kit
E01A0035 has been referenced in the below publications:
Dickkopf1 destabilizes atherosclerotic plaques and promotes plaque formation by inducing apoptosis of endothelial cells through activation of ER stress.
Brain Volumetrics, Regional Cortical Thickness and Radiographic Findings in Adults with Cyanotic Congenital Heart Disease.
Effect of super-flux polyethersulfone dialysis membrane on the removal of asymmetrical dimethylarginine: a pilot clinical study.
The Effect and Mechanism of Asymmetric Dimethylarginine Regulating Trophoblastic Autophagy on Fetal Growth Restriction.
The role and mechanism of asymmetric dimethylarginine in fetal growth restriction via interference with endothelial function and angiogenesis.