BlueGene Biotech Human Total β Amyloid Protein ELISA kit
E01A0039 Human Total β Amyloid Protein ELISA kit
Human Total β Amyloid Protein ELISA Kit is suitable for the detection of samples from human species. Total β Amyloid Protein can also be called Aβ, Aβ protein, Amyloid beta-peptide, Beta-amyloid peptide, Beta-amyloid protein, beta Amyloid Protein.
Specifications of Human Total β Amyloid Protein ELISA kit
Product Information
Cat. No.
E01A0039
Product Name
Human Alkaline Phosphatase ELISA kit
Species
Human
Product Size
48 Tests / 96 Tests
Concentration
5.0-100ng/mL
Sensitivity
1.0 ng/mL
Principal
Sandwich ELISA
Sample Volume
50 ul
Sample Type
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time
90 minutes
Platform
Microplate Reader
Conjugate
HRP
Detection Method
Colorimetric
Storage
2-8°C
Kit Components
MATERIALS
SPECIFICATION
QUANTITY
MICROTITER PLATE
96 wells
stripwell
ENZYME CONJUGATE
10 mL
1 vial
STANDARD A (0.5mL)
0 ng/mL
1 vial
STANDARD B (0.5mL)
5 ng/mL
1 vial
STANDARD C (0.5mL)
10 ng/mL
1 vial
STANDARD D (0.5mL)
25 ng/mL
1 vial
STANDARD E (0.5mL)
50 ng/mL
1 vial
STANDARD F (0.5mL)
100 ng/mL
1 vial
SUBSTRATE A
6 mL
1 vial
SUBSTRATE B
6 mL
1 vial
STOP SOLUTION
6 mL
1 vial
WASH SOLUTION (100 x)
10 mL
1 vial
BALANCE SOLUTION
3 mL
1 vial
Principle of the Assay
AΒ ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AΒ. Standards or samples are then added to the microtiter plate wells and AΒ if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AΒ present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AΒ are added to each well to “sandwich” the AΒ immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AΒ and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AΒ concentration in each sample is interpolated from this standard curve.a
Quality Control on Human Total β Amyloid Protein ELISA kit
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
Recovery
95-105%
Linearity
Diluent Ratio
Range %
1:2
92-102
1:4
91-107
1:8
91-105
Specificity/Cross-reactivity
No significant cross-reactivity or interference between AΒ and analogues was observed.
Citations of Human Total β Amyloid Protein ELISA kit
E01A0039 has been referenced in the below publications:
The study of the Characteristics of Cognitive Impairment after Acute Mild-to-Moderate Ischemic Stroke and the Role of SAA and Aβ in Cognitive Impairment.
