Insulin & Peptide Purification
Insulin and peptide purification is a critical process in the production of therapeutic peptides and proteins, particularly insulin for the treatment of diabetes. The purification aims to isolate and purify the target peptide or protein from a complex mixture of cellular components, impurities, and contaminants.
Types of Insulin & Peptide Purification
Chromatography Column
The Hanbon chromatographic column has a small overall footprint and is simple to use and maintain.
Chromatography System
Hanbon’s chromatography systems can be configured in a variety of ways and have dependable performance. They include the Bio-Lab® laboratory chromatography system and the Bio-Pro® pilot & process chromatography system, among others.
Buffer Dilution/Conditioning System
This approach eliminates the requirement for complex buffer solutions to achieve accuracy and consistency, reduces the number of storage tanks needed, minimizes labor and material requirements for dispensing, and ultimately lowers the production costs.
Filtration System
The Hanbon filtration system caters to biopharmaceutical clients of various scales and process routes. It offers a range of solutions, including the Bio-TFF tangential flow filtration system, depth filtration system, membrane cassette holders, and more. These options ensure suitability and flexibility for diverse biopharmaceutical applications.
Single-use System
Our offerings include a single-use chromatography system and a single-use tangential flow filtration system. These systems feature easily replaceable contact liquid components, ensuring a fast and convenient process.
Preparative Liquid Chromatography System
The separation and purification of pharmaceutical intermediates, biopharmaceuticals, synthetic medications, natural products, fine chemicals, food additives, and other sectors make substantial use of the Hanbon preparative liquid chromatography system.
Chromatographic Column
Hanbon medium and high pressure chromatography columns include laboratory and DAC (dynamic axial compression) columns. Hanbon, which also developed the DAC® (dynamic axial compression) column in 2005, has built the largest production and assembly plant for dynamic axial compression columns.
Can Insulin Be Purified From Animal Sources?
Yes, insulin can be purified from animal sources, such as porcine (pig) or bovine (cow) pancreas. Animal pancreases contain insulin in their beta cells. The purification process involves extracting the pancreatic tissue and then isolating and purifying the insulin from other pancreatic components.
The purification steps typically include techniques such as solvent extraction, chromatography, and crystallization. Solvent extraction helps separate the insulin from other pancreatic proteins and components. Chromatography techniques, such as reversed-phase chromatography or ion exchange chromatography, can be employed to further purify the insulin and remove impurities. Finally, the purified insulin can be crystallized to obtain a highly pure form.
It’s worth noting that the use of animal-derived insulin has decreased over the years with the advent of recombinant DNA technology, which allows for the production of insulin through genetically engineered organisms. Recombinant insulin is now the primary source of insulin for therapeutic use due to its purity, consistency, and reduced risk of transmitting diseases associated with animal-derived insulin.
