BlueGene Biotech Human Amyloid β Protein 40 ELISA kit
E01A0049 Human Amyloid β Protein 40 ELISA kit
Human Adenosine diphosphate ELISA kit is suitable for the detection of samples from human species. Amyloid β Protein 40 can also be called Aβ40, beta-amyloid 40.
Specifications of Human Amyloid β Protein 40 ELISA kit
Product Information
Cat. No.
E01A0049
Product Name
Human Amyloid β Protein 40 ELISA kit
Species
Human
Product Size
48 Tests / 96 Tests
Concentration
5.0-100ng/mL
Sensitivity
1.0 ng/mL
Principal
Sandwich ELISA
Sample Volume
50 ul
Sample Type
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time
90 minutes
Platform
Microplate Reader
Conjugate
HRP
Detection Method
Colorimetric
Storage
2-8°C
Kit Components
MATERIALS
SPECIFICATION
QUANTITY
MICROTITER PLATE
96 wells
stripwell
ENZYME CONJUGATE
10 mL
1 vial
STANDARD A (0.5mL)
0 ng/mL
1 vial
STANDARD B (0.5mL)
5 ng/mL
1 vial
STANDARD C (0.5mL)
10 ng/mL
1 vial
STANDARD D (0.5mL)
25 ng/mL
1 vial
STANDARD E (0.5mL)
50 ng/mL
1 vial
STANDARD F (0.5mL)
100 ng/mL
1 vial
SUBSTRATE A
6 mL
1 vial
SUBSTRATE B
6 mL
1 vial
STOP SOLUTION
6 mL
1 vial
WASH SOLUTION (100 x)
10 mL
1 vial
BALANCE SOLUTION
3 mL
1 vial
Principle of the Assay
AΒ40 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AΒ40. Standards or samples are then added to the microtiter plate wells and AΒ40 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AΒ40 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AΒ40 are added to each well to “sandwich” the AΒ40 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AΒ40 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AΒ40 concentration in each sample is interpolated from this standard curve.
Quality Control on Human Amyloid β Protein 40 ELISA kit
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
Recovery
92-105%
Linearity
Diluent Ratio
Range %
1:2
96-105
1:4
903-103
1:8
89-107
Specificity/Cross-reactivity
No significant cross-reactivity or interference between AΒ40 and analogues was observed.
Summary of the Assay Procedure for Human Amyloid β Protein 40 ELISA kit
Citations of Human Amyloid β Protein 40 ELISA kit
E01A0049 has been referenced in the below publications:
Therapeutic effect of probucol on mild cognitive impairment in type 2 diabetic patients.
Chronic Noise Exposure Acts Cumulatively to Exacerbate Alzheimer’s Disease-Like Amyloid-β Pathology and Neuroinflammation in the Rat Hippocampus.
