BlueGene Biotech Rat Epinephrine/Adrenaline ELISA kit
E02A0007 Rat Epinephrine/Adrenaline ELISA kit
Rat Epinephrine/Adrenaline ELISA kit is suitable for the detection of samples from Rat species. Epinephrine/Adrenaline can also be called ADR, or EPI.
Specifications of Rat Epinephrine/Adrenaline ELISA kit
Product Information
Cat. No.
E02A0007
Product Name
Rat Epinephrine/Adrenaline ELISA kit
Species
Rat
Product Size
48 Tests / 96 Tests
Concentration
100-2500pg/ml
Sensitivity
1.0 pg/mL
Principal
Competitive ELISA
Sample Volume
100 ul
Sample Type
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time
90 minutes
Platform
Microplate Reader
Conjugate
HRP
Detection Method
Colorimetric
Storage
2-8°C
Kit Components
MATERIALS
SPECIFICATION
QUANTITY
MICROTITER PLATE
96 wells
stripwell
ENZYME CONJUGATE
6.0 mL
1 vial
STANDARD A (0.5mL)
0 pg/mL
1 vial
STANDARD B (0.5mL)
100 pg/mL
1 vial
STANDARD C (0.5mL)
250 pg/mL
1 vial
STANDARD D (0.5mL)
500 pg/mL
1 vial
STANDARD E (0.5mL)
1000 pg/mL
1 vial
STANDARD F (0.5mL)
2500 pg/mL
1 vial
SUBSTRATE A
6 mL
1 vial
SUBSTRATE B
6 mL
1 vial
STOP SOLUTION
6 mL
1 vial
WASH SOLUTION (100 x)
10 mL
1 vial
BALANCE SOLUTION
3 mL
1 vial
Principle of the Assay
EPI ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-EPI antibody and an EPI-HRP conjugate. The assay sample and buffer are incubated together with EPI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EPI concentration since EPI from samples and EPI-HRP conjugate compete for the anti-EPI antibody binding site. Since the number of sites is limited, as more sites are occupied by EPI from the sample, fewer sites are left to bind EPI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EPI concentration in each sample is interpolated from this standard curve.
Quality Control on Rat Epinephrine/Adrenaline ELISA kit
Coefficient of Variance
Intra Variation% <10%
Inter Variation% <12%
Recovery
94-109%
Linearity
Diluent Ratio
Range %
1:2
95-104
1:4
93-106
1:8
90-110
Specificity/Cross-reactivity
No significant cross-reactivity or interference between EPI and analogues was observed.
Summary of the Assay Procedure for Rat Epinephrine/Adrenaline ELISA kit
Citations of Rat Epinephrine/Adrenaline ELISA kit
E02A0007 has been referenced in the below publications:
Role of plateau hypoxia hypothermia complex damage to the vascular endothelial cells and the protective effect.
The mechanism research of neurons injury in the basolateral of amygdala induced by stress.