BlueGene Biotech Rat Active Caspase 3 ELISA kit
E02A0015 Rat Active Caspase 3 ELISA kit
Rat Active caspase 3 ELISA kit is suitable for the detection of samples from Rat species. Active caspase 3 can also be called CPP32, CPP32B, SCA1, Apoptain, Yama, Apoptosis-Related Cysteine Peptidase, Cysteinyl Aspartate Specific Proteinases 3, SREBP cleavage activity 1, Active casp3, casp3, casp-3, casp 3.
Specifications of Rat Active caspase 3 ELISA kit
Product Information
Cat. No.
E02A0015
Product Name
Rat Active caspase 3 ELISA kit
Species
Rat
Product Size
48 Tests / 96 Tests
Concentration
5.0-100ng/ml
Sensitivity
1.0 ng/mL
Principal
Sandwich ELISA
Sample Volume
50 ul
Sample Type
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time
90 minutes
Platform
Microplate Reader
Conjugate
HRP
Detection Method
Colorimetric
Storage
2-8°C
Kit Components
MATERIALS
SPECIFICATION
QUANTITY
MICROTITER PLATE
96 wells
stripwell
ENZYME CONJUGATE
10 mL
1 vial
STANDARD A (0.5mL)
0 ng/mL
1 vial
STANDARD B (0.5mL)
5.0 ng/mL
1 vial
STANDARD C (0.5mL)
10 ng/mL
1 vial
STANDARD D (0.5mL)
25 ng/mL
1 vial
STANDARD E (0.5mL)
50 ng/mL
1 vial
STANDARD F (0.5mL)
100 ng/mL
1 vial
SUBSTRATE A
6 mL
1 vial
SUBSTRATE B
6 mL
1 vial
STOP SOLUTION
6 mL
1 vial
WASH SOLUTION (100 x)
10 mL
1 vial
BALANCE SOLUTION
3 mL
1 vial
Principle of the Assay
ACTIVE CASPASE 3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ACTIVE CASPASE 3. Standards or samples are then added to the microtiter plate wells and ACTIVE CASPASE 3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ACTIVE CASPASE 3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ACTIVE CASPASE 3 are added to each well to “sandwich” the ACTIVE CASPASE 3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ACTIVE CASPASE 3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACTIVE CASPASE 3 concentration in each sample is interpolated from this standard curve.
Quality Control on Rat Active caspase 3 ELISA kit
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
Recovery
92-108%
Linearity
Diluent Ratio
Range %
1:2
95-105
1:4
93-104
1:8
89-109
Specificity/Cross-reactivity
No significant cross-reactivity or interference between Active CASP3 and analogues was observed.
Summary of the Assay Procedure for Rat Active caspase 3 ELISA kit
Citations of Rat Active caspase 3 ELISA kit
E02A0015 has been referenced in the below publications:
Hypoxic Preconditioning Augments the Therapeutic Efficacy of Bone Marrow Stromal Cells in a Rat Ischemic Stroke Model.
