BlueGene Biotech Rat Alpha Amylase ELISA kit
E02A0010 Rat Alpha Amylase ELISA kit
Rat Alpha Amylase ELISA kit is suitable for the detection of samples from Rat species. Alpha Amylase can also be called glycogenase, α amylase, endoamylase, Taka-amylase A, 1,4-α-D-glucan glucanohydrolase, AMY.
Specifications of Rat Alpha Amylase ELISA kit
Product Information
Cat. No.
E02A0010
Product Name
Rat Alpha Amylase ELISA kit
Species
Rat
Product Size
48 Tests / 96 Tests
Concentration
10-250ng/ml
Sensitivity
1.0 ng/mL
Principal
Competitive ELISA
Sample Volume
100 ul
Sample Type
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time
90 minutes
Platform
Microplate Reader
Conjugate
HRP
Detection Method
Colorimetric
Storage
2-8°C
Kit Components
MATERIALS
SPECIFICATION
QUANTITY
MICROTITER PLATE
96 wells
stripwell
ENZYME CONJUGATE
6.0 mL
1 vial
STANDARD A (0.5mL)
0 ng/mL
1 vial
STANDARD B (0.5mL)
10 ng/mL
1 vial
STANDARD C (0.5mL)
25 ng/mL
1 vial
STANDARD D (0.5mL)
50 ng/mL
1 vial
STANDARD E (0.5mL)
100 ng/mL
1 vial
STANDARD F (0.5mL)
250 ng/mL
1 vial
SUBSTRATE A
6 mL
1 vial
SUBSTRATE B
6 mL
1 vial
STOP SOLUTION
6 mL
1 vial
WASH SOLUTION (100 x)
10 mL
1 vial
BALANCE SOLUTION
3 mL
1 vial
Principle of the Assay
AMY ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AMY antibody and an AMY-HRP conjugate. The assay sample and buffer are incubated together with AMY-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMY concentration since AMY from samples and AMY-HRP conjugate compete for the anti-AMY antibody binding site. Since the number of sites is limited, as more sites are occupied by AMY from the sample, fewer sites are left to bind AMY-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMY concentration in each sample is interpolated from this standard curve.
Quality Control on Rat Alpha Amylase ELISA kit
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
Recovery
94-106%
Linearity
Diluent Ratio
Range %
1:2
95-103
1:4
93-108
1:8
90-112
Specificity/Cross-reactivity
No significant cross-reactivity or interference between AMY and analogues was observed.
Summary of the Assay Procedure for Rat Alpha Amylase ELISA kit
Citations of Rat Alpha Amylase ELISA kit
E02A0010 has been referenced in the below publications:
The protective effect and mechanism of the alanyl-glutamine dipeptide in the treatment of severe acute pancreatitis.
