Yeast Surface Display Platform

Our YSD platform is based on the α-agglutinin display system. In this system, the antibody fragments or peptides gene sequence is fused to the Aga2p subunit, which is secreted and anchored to the yeast cell wall via disulfide bonds to the Aga1p subunit. Upon induction with galactose, the fusion protein is expressed and displayed on the yeast cell surface, enabling direct interaction with fluorescently labeled antigens during screening. This design allows efficient display of functional VHH molecules for downstream screening and affinity analysis.

Yeast Surface Display Platform Advantages

Compared with other discovery technologies, yeast display offers several key advantages:

Direct linkage between genotype and phenotype

Eukaryotic expression system enabling proper protein folding

High display density (10⁴–10⁵ copies per cell)

Quantitative and tunable selection using FACS

Multi-parameter screening (affinity, specificity, epitope competition)

Reduced screening bias and lower false-positive rates

Compatibility with both NGS and monoclonal (Sanger-based) analysis

Applications and Molecule Formats

Our YSD Platform supports the display and screening of various biomolecules, including: Nanobodies (VHH), Single-chain antibodies (scFv) ,Fab fragments, Peptides, and other proteins.

For more details please contact NBbiolab